A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
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A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
The enzymatic hydrolysis of cellulose and lignocellulosic materials is marked by a rate decrease along the reaction time. Cellobiohydrolase slow dissociation from the substrate and its inhibition by the cellobiose produced are relevant factors associated to the rate decrease. In that sense, addition of β-glucosidases to the enzyme cocktails employed in cellulose enzymatic hydrolysis not only pr...
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The high cellulase enzyme dosages required for hydrolysis of cellulose is a major cost challenge in lignocellulosic ethanol production. One method to decrease the enzyme dosage and increase biocatalytic productivity is to re-use β-glucosidase (BG) via immobilization. In the present research, glutaraldehyde cross-linked BG was entrapped in calcium alginate gel particles. More than 60% of the enz...
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Väljamäe, P. 2002. The kinetics of cellulose enzymatic hydrolysis: implications of the synergism between enzymes. Acta Universitatis Upsaliensis. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology 781. 54 pp. Uppsala. ISBN 91-554-5479-8. The hydrolysis kinetics of bacterial cellulose and its derivatives by Trichoderma reesei cellulases was studied. The c...
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ژورنال
عنوان ژورنال: Biochemistry and Biophysics Reports
سال: 2016
ISSN: 2405-5808
DOI: 10.1016/j.bbrep.2016.05.014